Record the temperature of the room, the ice bath and the hot water in the table. unknown solution is 5 at temperature of 23C. Remove the strip and promptly compare the results to the specimen colors indicated on the dispenser. 0000001893 00000 n
Tear a few leaves of red cabbage into small pieces and place the leaves into a 500-mL beaker. The precision and accuracy of the method can be improved by (i) fitting the buret with an Comment on the shape of the curve. This, data will help you establish a key for a cabbage indicator. Zero is the most acidic, and 14 is the most basic while 7 is the neutral number for example water. Thus, in order to maintain homeostasis and survive, organisms must maintain a stable internal pH. 6!Q(
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Make sure that all of the leaf pieces are completely submerged. What is an acidic solution? An excellent price as well. pH meters are used to measure pH values of water samples, such as determining acidity of rainwater samples. Acid is listed on average in three forms on a nutrition label for soda. spam or irrelevant messages, We use cookies to give you the best experience possible. A laboratory pH meter typically has a special probe capped with a membrane that is sensitive to \(\ce{H^+}\) ion concentrations. avoid running a washing machine with a small load, add additional insulation on a home hot water heater. pH Table: Here we see the pH of the water, NaOH and HCL. \[\text{pH value} = X \ce{->} [\ce{H^+}] = 10^{-X} M\]. endstream
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Add 10 drops of hydrogen peroxide to the tube marked A. In particular, you will be examining the effects of these environmental factors on the ability of catalase to convert H2O2 into H2O and O2. Materials: For this lab we need pH paper, a spot plate, water (distilled, if available), and substances with varying pH. PROCEDURES CALIBRATION OF pH METER The scientific method is a systematic approach to problem solving. Calculate the [\(\ce{H^+}\)] for both solutions, include units in your answer: For pH 10, [\(\ce{H^+}\)] = _________________, For pH 6, [\(\ce{H^+}\)] = _________________. *You can also browse our support articles here >. By continuing well assume youre on board with our cookie policy, Dont waste Your Time Searching For a Sample, Accuracy And Precision Lab Report Discussion, Acid Base Titration Lab Report Discussion, Standardization Of Potassium Permanganate Lab Report Discussion, ASK writer for The result from the experiment of determining the pH of an unknown solution is 5.57 at temperature of 23.9. 0000003990 00000 n
Problem: How could certain factors of pH and Temperature could affect enzymes? The neutral pH is at 7 while less than 7 is acidic and more than 7 is base. Think-Pair-Share and Whip-Around). Record the data in each test tube in the table. Soapy Water 8 Conclusion In concluding this lab I found that, in general most groups had similar recordings in their lab. Add 3 mL of hydrogen peroxide to each test tube. Weblia.erc.gov.ph 1 / 4. Put potato juice in 3 test tubes labeled B. 0000002371 00000 n
As a precaution, the pH meter is made sure to be frequently calibrated. Frequent calibration will witheld coating and aging of the electrode. 13. %PDF-1.3 The result from the experiment of determining the pH of an A buffer is a solution whose pH resists change on addition of small amounts of either an acid or a base. We never tested the pH and we never used NaOH or HCL in the lab. [K4] These should be restructured to if-then format. WebThe pH chart, conversely, does not allow for the determination of pH values smaller than one unit. But for our purposes, we will differentiate each of these steps in our applications of the scientific method. Here you can choose which regional hub you wish to view, providing you with the most relevant information we have for your specific region. The report describes the experiment from the start to end. Explain that pH is relevant to the garden because it indicates nutrient availability. The buffering capacity of a solution is tested by adding small amounts of acid (for example, \(\ce{HCl}\)) and base (for example, \(\ce{NaOH}\)) and checking the pH after each addition. However, before e
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The electrode is then, Clinical Medicine (Parveen J. Kumar; Michael L. Clark), Diseases of Ear, Nose and Throat (P L Dhingra; Shruti Dhingra), Apley's System of Orthopaedics and Fractures, Ninth Edition (Louis Solomon; David Warwick; Selvadurai Nayagam), Oxford Handbook of Clinical Medicine (Murray Longmore; Ian Wilkinson; Andrew Baldwin; Elizabeth Wallin), Clinical Examination: a Systematic Guide to Physical Diagnosis (Nicholas J. Talley; Simon O'Connor), Apley's Concise System of Orthopaedics and Fractures, Third Edition (Louis Solomon; David J. Warwick; Selvadurai Nayagam), Shigley's Mechanical Engineering Design (Richard Budynas; Keith Nisbett), Little and Falace's Dental Management of the Medically Compromised Patient (James W. Little; Donald Falace; Craig Miller; Nelson L. Rhodus), Essential Surgery (Clive R. G. Quick; Joanna B. Reed), Gynaecology by Ten Teachers (Louise Kenny; Helen Bickerstaff), Browse's Introduction to the Symptoms and Signs of Surgical Disease (John Black; Kevin Burnand), Law of Torts in Malaysia (Norchaya Talib), Lecture Notes: Ophthalmology (Bruce James; Bron), A simple example for lab report reference, Quiz Chemistry Introduction to Chemistry (chemical). Acids damage living tissue by causing burns upon contact. 0000003497 00000 n
Many commercial products use pH as an advertisement tool, such as shampoo and water; more recently, food and drink of certain pH has been touted as more healthful. Put the second test tube in the ice bath. Remember to answer the specific question that had been asked in the assignment. Leave the first test tube in the test tube rack. Chemistry questions and answers. Wait 5 minutes and record the height of oxygen bubbles in each test tube. WebPH paper (litmus paper) determines how acidic or how basic a substance is. From the objectives of this lab, our questions are as follows: Based on the questions above, come up with some possible hypotheses. An excess of \(\ce{H^+}\) or \(\ce{OH^-}\) can interfere with the functioning of biological molecules, especially proteins. The solution is placed below Save the things that you have done above. WebConclusion: - Based on the data results the hypothesis constructed was correct. The active site of an enzyme recognizes, confines, and orients the substrate in a particular direction. process is paused. indicator, then you can use those values to calibrate a cabbage pH indicator. 0000007343 00000 n
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How does pH affect the ability of enzymes to catalyze chemical reactions? WebIn a pH titration you measure the pH as a function of the volume of titrant added and determine the equivalence point as the point in where there is an inflection in the slope of the curve. The procedure for operating every pH meter is slightly different. Enzymes are substrate specific, meaning that they catalyze only specific reactions. The sample is removed and the The indicator used in this lab will be obtained from a natural source, red cabbage. electrode is rinsed with distilled water. Table A: Control Solutions (in order from most acidic to most basic), Lemon Juice Hand Sanitizer Soap/Dishwashing This suffix indicates that a molecule is an enzyme. Below are 5 core components of a good conclusion for any scientific lab report: 1. The pH The acids and bases shown below cover a range of pH values. This page titled 8: Acid, Bases and pH (Experiment) is shared under a CC BY-NC license and was authored, remixed, and/or curated by Santa Monica College. submerged into the buffer solution with pH of 7. The pH indicator determines the measurements of the pH range of acids and bases and those Retrieved from https://paperap.com/paper-on-ph-lab-report-testing-household-liquids/. Part B: Cold Temperature and Hot Temperature. maintain its pH when a small amount of strong acid or The reading is recorded. The result of determining the pH of an unknown solution is Alice Waters Institute for Edible Education. Plug the probe into one port on the side of the pH meter. A strong conclusion should briefly describe the methods used in the experiment. Light Shield and Sample cell Method The turbidimeter was allowed to warm up for approximately 110 minutes. Then, 20 drops were added and gently swirling the beaker to mix the solution and the hydrochloric acid and wait until the pH meter dropped 1. HT0+QZi{:@ C !R%lyFZ' `~* Students will record the colors of the pH standards. solution. Amazing writer! HM0:*3H Ro;#1wQ:c]{uA~vUl~CPoav7 1VXDP>i?=9? Wa?m/{uLa;j/d_y{,SJ`|R4y VLY4m/C+=^'OA}]4oaDgD#V1kU4<4'.h:GA6udv!>.QyR)RiucI+ZuZW$lKb]REY9gf]e~tZS|,i
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Each group should have: Each group of 2-3 students will test a sample. Cross). Hypothesis- If you can measure the pH of a range of acids and bases with a universal pH. A pH value is a number, usually between 0 and 14, that represents the acidity or basicity of a solution. Make sure your instructor checks your procedures before you continue. Solutions that have a high pH level or above 7 are considered basic. WebCharles Law Lab Report Conclusion Charles law lab report Your Homework Help vvgb law com May 14th, 2018 - Of Gas Law Constant Lab Report Charles s law Works enough can gasp in neither narrative you teaches have and awe charles law lab report The conclusion of Lab Report Gas Laws Short Paragraph Weblia.erc.gov.ph 1 / 4. If [H+1] = 5 x 10-2 M, the pH is 1.3. It is the stage where all other stages are premised. In the second test tube, add 5 drops of NaOH.
The catalase sample for todays lab will be from a potato. A reaction happens because chemical bonds are broken and atoms are rearranged. WebChemistry. WebpH: pH could affect the activity of the enzyme (catalase). What might explain your results? Ask students to identify their samples as acidic or basic. Clearly, NaOH is very basic and the HCL is very acidic and the water was slightly acidic, but being closer to neutral. Gently boil the mixture on heating plate until it appears dark purple in color (5-10 min). Create job board, listing garden jobs and pH lab.
This is proven with our, findings when we mixed Lemon juice with the cabbage water it turned red indicating a low pH, Conclusion- In conclusion, if you compare the worldwide pH indicator to the cabbage water one, there are some color differences but using this method to test the pHs of different substances, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Purpose- Explore acids and bases using 2 dif, data will help you establish a key for a cabbage indicator, everyday solutions with this new cabbage indicator, Civilization and its Discontents (Sigmund Freud), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Give Me Liberty! On one beaker, measure the distilled water with the pH meter and record the pH level. Rinse the tip of the pH pen with tap water between tests. The room temperature was 20 degrees Celsius, the Ice Bath was 0 degrees Celsius and the Hot Water was 90 degrees Celsius. If your results are different from your hypothesis, why might they differ? CT{3d_ vz
A pH lab report conclusion is a summary of the results and findings of a pH experiment. What is the effect of substrate concentration on the rate of enzyme catalyzed reactions? Other than that, the rest of 0000003574 00000 n
This tells us that the pH of our unknown solution is greater than or equal to 2 because methyl violet turns violet at pH values of 2 or greater. Bases have (high OR low) pH, and (high OR low) \(\ce{H^+}\) ion concentration. CONCLUSION In conclusion, the pH meter is calibrated with using three WebApril 15th, 2018 - Lab report museum Monday 11 August 2008 Report 5 Meiosis in angiosperm microsporogenesis INTRODUCTION CONCLUSION As a conclusion GUIDELINES FOR WRITING LAB REPORTS Marietta College April 27th, 2018 - Lab Report Guidelines Page lrg 1 GUIDELINES FOR Conclusions Explanation of Results Estimating the relative length very clearly explained and easy to follow, Finding Equilibrium Conc from Kc without all of the equilibrium equations Quadratic Eq, Community Health and Population-Focused Nursing Field Experience (C229), Basic News Writing Skills 8/23-10/11Fnl10/13 (COMM 160), Advanced Concepts in Applied Behavior Analysis (PSY7709), (BUSA 1101), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Chapter 4 - Summary Give Me Liberty! HTM0Wm6]aK)C?Trqf!GpF2N@0\L*Q'pM@$h!DgVlGm/MM3-,Ol>Fyxz`8}#x\G>KP)f4>(KaE y~1a];8\&F(H`]GmXZiWn+sXFOxM Aj http://opencourselibrary.org/biol-160-general-biology-with-lab/, define the following terms: metabolism, reactant, product, substrate, enzyme, denature, describe what the active site of an enzyme is (be sure to include information regarding the relationship of the active site to the substrate), describe the specific action of the enzyme catalase, include the substrate and products of the reaction, list what organelle catalase can be found in every plant or animal cell, list the factors that can affect the rate of a chemical reaction and enzyme activity, explain why enzymes have an optimal pH and temperature to ensure greatest activity (greatest functioning) of the enzyme (be sure to consider how virtually all enzymes are proteins and the impact that temperature and pH may have on protein function), explain why the same type of chemical reaction performed at different temperatures revealed different results/enzyme activity, explain why warm temperatures (but not boiling) typically promote enzyme activity but cold temperature typically, explain why increasing enzyme concentration promotes enzyme activity, explain why the optimal pH of a particular enzyme promotes its activity, if given the optimal conditions for a particular enzyme, indicate which experimental conditions using that particular enzyme would show the greatest and least enzyme activity. Results: pH 7: Rate of O2formation and keep the result accurate. The scale has to do with the amount of hydrogen ions [resent in a substance. A pH value is a number, usually between 0 and 14, that represents the acidity or basicity of a solution. endstream
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in a storage solution. HTP=O0+`---Ol~zO 9VWd&ph4B~xa;dOSFWF5(h[p8 When the pH is lower the H+ ion in the solution is high. Tell them that the ideal acidity level for soil is between 6 and 7. When carrying the experiment, students must also make sure to rinse the electrode with distilled water before and after immersing it into any solutions. pH is defined by the equation: pH = -log[H+1] (1) where the logarithm is taken to the base 10. 0000002451 00000 n
HV{TW3y4UE-hIxF(KD|l]I c HhQ,he|RZmA>jEE+*V-z->Z]9jE@s9 +(xU Pi{;*Lc The color may be between the pH standard colors (e.g. Copyright 2003 - 2023 - UKEssays is a trading name of Business Bliss Consultants FZE, a company registered in United Arab Emirates. Unknown solution X, is prepared. After 5 minutes measure the height of the oxygen bubble in each test tube. The paper changes color accordingly to color code on the pH scale. Values between 7 and 14 indicate a basic solution. `F:q_`$p>(#6 WebApril 15th, 2018 - Lab report museum Monday 11 August 2008 Report 5 Meiosis in angiosperm microsporogenesis INTRODUCTION CONCLUSION As a conclusion GUIDELINES FOR WRITING LAB REPORTS Marietta College April 27th, 2018 - Lab Report Guidelines Page lrg 1 GUIDELINES FOR Conclusions Explanation of Results Estimating the relative length help. Record each in the table. The pH of rain in parts of the U.S. is less than pH 5, which is harmful to aquatic life and human health. Add a drop or two or bromcresol green indicator to each of these solutions. Weblia.erc.gov.ph 1 / 6. The tomato juice will be acidic; the distilled water will be neutral; the Windex will be basic; the vinegar will be basic; the soda will be basic; the milk will be neutral; the buttermilk will be neutral; the baking soda solutions will be acidic; the green cleaner will be basic; the household cleaner will be acidic; the Lemon Juice will be acidic. Note: Do not completely submerge the probe. It was first isolated in 1928 by the Hungarian-born scientist Szent-Gyorgi and structurally characterized by Haworth in 1933. Extrapolate curve to pH 7 and measure the ml. Any scientific information contained within this essay should not be treated as fact, this content is to be used for educational purposes only and may contain factual inaccuracies or be out of date. Include and Analyze Final Data A lab report should include a conclusion that analyzes the final data. pH indicators are a good way to easily and quickly show the approximate pH by color when compared to a standard. Basic solutions are also described in terms of pH. [K2] You need to introduce the specific reaction we studied in the introduction. Unknown solution X. %%EOF
Record your data in the table. WebConclusion This study demonstrates that the titration method relying on visual detection of the end as illustrated in this report: cNaOH =98.3 0.7 mM and cunknown =62.00.5 mM (confidence level 95%). Ph Lab Report Discussion Get High-quality Paper helping students since 2016 Materials: For this lab we need pH paper, a spot plate, water (distilled, if available), The calibration is started of with buffer Label the sites and dig holes where samples will be collected. It should also consider any limitations of the experiment and suggest areas for further research. Some chemical bonds are stronger than others. )!'Q?sm[{cOR)hpM^\oy?6~yUv^i31tLL-5xEQiH{U4|]01FVoD"V#j|
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In this lab you measure the PH levels of multiple liquids. The pH scale range from 1 to 14. This may include discussing the practical applications of the findings or suggesting areas for further research. My hypothesis was basically that pH and temperature can affect the enzyme because if the environment is different to what it is regularly use to it could have different affect on it. WebHorseradish Peroxidase Lab Report To catalyze a reaction, an enzyme will grab on (bind) to one or more reactant molecules. Copy the Soil pH Lab worksheet to hand out. This is less surprising when reviewing the amount of acid in soda. Pure water is usually pH 7. At the conclusion of the lab, the student should be able to: Hydrogen peroxide is a toxic product of many chemical reactions that occur in living things. different buffer solutions with pH of 4,7 and 10. Remember to use clear and concise language, and to avoid getting bogged down in the details. 0000009106 00000 n
You will be use them to write a lab report a little later in the course. Personal Protective Equipment (PPE) required: safety goggles, lab coat, closed-toe shoes Materials and Equipment. The pH of dehydrating. { "01:_Measurements_in_the_Laboratory_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "02:_Paper_Chromatography_of_Gel_Ink_Pens_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "03:_The_Properties_of_Oxygen_Gas_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "04:_Detection_and_Absorption_of_Ultraviolet_Light_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "05:_Flame_Tests_and_Atomic_Spectra_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "06:_Lewis_Structures_and_Molecular_Shapes_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "07:_Electrical_Conductivity_of_Aqueous_Solutions_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "08:_Acid_Bases_and_pH_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "09:_Single_Replacement_Reactions_and_Batteries_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "10:_Double_Replacement_Reactions_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "11:_Synthetic_Polymers_and_Plastics_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "12:_Making_Soap_-_Saponification_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, { Chem_10_Experiments : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Chem_11_Experiments : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Chem_12_Experiments : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Chem_9_Experiments : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, [ "article:topic", "authorname:smu", "showtoc:no", "license:ccbync" ], https://chem.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fchem.libretexts.org%2FAncillary_Materials%2FLaboratory_Experiments%2FWet_Lab_Experiments%2FGeneral_Chemistry_Labs%2FOnline_Chemistry_Lab_Manual%2FChem_9_Experiments%2F08%253A_Acid_Bases_and_pH_(Experiment), \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), 7: Electrical Conductivity of Aqueous Solutions (Experiment), 9: Single Replacement Reactions and Batteries (Experiment), Part A: Preparing pH indicator and pH standards - INSTRUCTOR DEMO, Part B: Qualitative Analysis for pH Values of Everyday Chemicals, Part C: Quantitative Analysis for pH Values of Everyday Chemicals, Part A: Color of Red Cabbage Indicator with pH standards, status page at https://status.libretexts.org, To set up and show how to use a pH indicator, To understand pH differences of acids and bases, To understand relationship between pH and H+ ion concentration. The functionality of an enzyme is determined by the shape of the enzyme. Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. Buffer solution with standardized pH of 7 t7tsx^4cbMDMEw(z']6BNJXJ.},LG:(45l,M]!"03@raFyT ]5+o?)C=|u')oY$zE\rVG==MF25tQC|U>o]mmV' xX
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{X9deU]nCIb-4~@ iuQlR@O%=! I just still dont understand where the NaOH and HCL came from. This is a way to determine pH of a solution visually. immersing it into any solutions. you Using the whiteboard at the front of the Ramada, collect data from each group. After this lesson, students will be able to: Welcome students and introduce the Soil pH Lab. You should see a pH value reading. Living organisms are very sensitive to the effects of acids and bases in their environment. Record the pH value (to 0.01 pH) shown on the pH meter screen. Found that, in order to maintain homeostasis and survive, organisms must maintain a stable internal pH in...: rate of O2formation and keep the result of determining the pH level above. Just still dont understand where the NaOH and HCL came from and in! A small amount of acid in soda into one port on the rate of O2formation and the. 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